Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- NBP2-37257 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal HOXB4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Membrane permeabilizing amphiphilic peptide delivers recombinant transcription factor and CRISPR-Cas9/Cpf1 ribonucleoproteins in hard-to-modify cells.
Del'Guidice T, Lepetit-Stoffaes JP, Bordeleau LJ, Roberge J, Théberge V, Lauvaux C, Barbeau X, Trottier J, Dave V, Roy DC, Gaillet B, Garnier A, Guay D
PloS one 2018;13(4):e0195558
PloS one 2018;13(4):e0195558
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HOXB4 Antibody (3A2F2) [NBP2-37257] - Western blot analysis using HOXB4 mAb against human HOXB4 (AA: 16-251) recombinant protein. (Expected MW is 51.9 kDa)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HOXB4 Antibody (3A2F2) [NBP2-37257] - Western blot analysis using HOXB4 mAb against HEK293 (1) and HOXB4 (AA: 16-251)-hIgGFc transfected HEK293 (2) cell lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: HOXB4 Antibody (3A2F2) [NBP2-37257] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HOXB4 Antibody (3A2F2) [NBP2-37257] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using HOXB4 mouse mAb with DAB staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HOXB4 Antibody (3A2F2) [NBP2-37257] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using HOXB4 mouse mAb with DAB staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunomicroscopy: HOXB4 Antibody (3A2F2) [NBP2-37257] - HeLa cells after 1 minute co-incubation using HoxB4 (7 micro M) and 6His-CM18-PTD4 (10 micro M) in fluorescent microscopy analysis. Prior to immune-labeling, cells were permeabilized and fixed with a primary mouse anti-HOXB4 monoclonal antibody (3A2F2). Scale Bars: 50 micro m. Citation: Del'Guidice T, Lepetit-Stoffaes J-P, Bordeleau L-J, Roberge J, Theberge V, Lauvaux C, et al. (2018) Membrane permeabilizing amphiphilic peptide delivers recombinant transcription factor and CRISPR-Cas9/Cpf1 ribonucleoproteins in hard-to-modify cells. PLoS ONE 13(4): e0195558. https://doi.org/10.1371/journal.pone.0195558