MA3-075

antibody from Invitrogen Antibodies

Targeting: TAF4 TAF2C, TAF2C1, TAF4A, TAFII130, TAFII135

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA3-075

Provider

Invitrogen Antibodies

Product name

TAF4 Monoclonal Antibody (20TA-1B12)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

MA3-075 detects TATA-Binding Protein (TBP) Associated factor 4 from human and mouse samples. MA3-075 has been successfully used in Western blot and immunofluorescence applications. The MA3-075 immunogen is a recombinant TAF4 protein. Epitope is localized between aa 815 and 836.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

20TA-1B12

Vial size

50 µL

Concentration

Conc. Not Determined

Storage

-20° C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of TAF4 in HeLa cells. The cells were fixed with 4% paraformaldehyde in PBS for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a TAF4 mouse monoclonal antibody (Product # MA3-075) at a dilution of 1:4000 in blocking buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:1000 for at least 30 minutes at a room temperature in the dark (green). Nuclei (blue) were stained with Hoechst 33342 (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.