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LearnPA5-104475
antibody from Invitrogen Antibodies
Targeting: TAF4
TAF2C, TAF2C1, TAF4A, TAFII130, TAFII135
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PA5-104475 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TAF4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Antibody detects endogenous levels of total TAF4.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TAF4 in extracts from Mouse brain. Samples were incubated with TAF4 polyclonal antibody (Product # PA5-104475). The lane on the left was treated with blocking peptide.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TAF4 in various samples (Lane 1: mouse lung lysate, Lane 2: rat heart lysate, Lane 3: rat heart lysates treated with blocking peptide). Samples were incubated with TAF4 polyclonal antibody (Product # PA5-104475).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TAF4 in NIH-3T3. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with TAF4 polyclonal antibody (Product # PA5-104475) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
