Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Other assay [1]
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- Product number
- 14-1206-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-IL-20RB Monoclonal Antibody (20RNTC), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 20RNTC monoclonal antibody recognizes mouse and human IL-20R2 (interleukin-20 receptor 2, IL-20Rb) in either of its two heterodimeric complexes formed by pairing with IL-20R1 or IL-22R1. IL-20R2 belongs to the class II cytokine receptor family that shares a characteristic pattern of cysteine, proline and tryptophan residues in the extracellular domain. IL-20R2 paired with IL-20R1 forms a receptor that binds IL-20, IL-19, or IL-24. IL-20R2 paired with IL22R1 forms a receptor that binds IL-20 or IL-24. The IL-20R complex signals primarily via the JAK-STAT pathway. IL-20R2 is found predominantly on cells of epithelial origin such as gut and skin and can be upregulated during inflammation. Immunofluorescent staining of A375 cells reveals a punctate staining pattern at the cell membrane, as well as diffuse, cytoplasmic staining that is likely associated with protein synthesis and transport in the endoplasmic reticulum and golgi. Applications Reported: This 20RNTC antibody has been reported for use in immunocytochemistry, immunoblotting (WB) and flow cytometric analysis. Applications Tested: This 20RNTC antibody has been tested by immunofluorescent staining and flow cytometric analysis of fixed and permeabilized A375 cells. This can be used between 1-10 µg/mL. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human, Mouse
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- 20RNTC
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references IL-24 Promotes Pseudomonas aeruginosa Keratitis in C57BL/6 Mouse Corneas.
Interleukin-19 acts as a negative autocrine regulator of activated microglia.
Signaling via the IL-20 receptor inhibits cutaneous production of IL-1β and IL-17A to promote infection with methicillin-resistant Staphylococcus aureus.
Interleukin (IL)-19, IL-20 and IL-24 are produced by and act on keratinocytes and are distinct from classical ILs.
Ross BX, Gao N, Cui X, Standiford TJ, Xu J, Yu FX
Journal of immunology (Baltimore, Md. : 1950) 2017 May 1;198(9):3536-3547
Journal of immunology (Baltimore, Md. : 1950) 2017 May 1;198(9):3536-3547
Interleukin-19 acts as a negative autocrine regulator of activated microglia.
Horiuchi H, Parajuli B, Wang Y, Azuma YT, Mizuno T, Takeuchi H, Suzumura A
PloS one 2015;10(3):e0118640
PloS one 2015;10(3):e0118640
Signaling via the IL-20 receptor inhibits cutaneous production of IL-1β and IL-17A to promote infection with methicillin-resistant Staphylococcus aureus.
Myles IA, Fontecilla NM, Valdez PA, Vithayathil PJ, Naik S, Belkaid Y, Ouyang W, Datta SK
Nature immunology 2013 Aug;14(8):804-11
Nature immunology 2013 Aug;14(8):804-11
Interleukin (IL)-19, IL-20 and IL-24 are produced by and act on keratinocytes and are distinct from classical ILs.
Kunz S, Wolk K, Witte E, Witte K, Doecke WD, Volk HD, Sterry W, Asadullah K, Sabat R
Experimental dermatology 2006 Dec;15(12):991-1004
Experimental dermatology 2006 Dec;15(12):991-1004
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunofluorescent microscopy of fixed and permeabilized A375 cells using 10 µg/mL of Anti-Human/Mouse IL-20 Receptor 2 (IL-20Rb) Purified (right) or Rat IgG2a kappa Isotype Control Purified (Product # 14-4321-82) (left) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and then Streptavidin FITC (Product # 11-4317-87).