MAB8405

antibody from Novus Biologicals

Targeting: TSPAN33 MGC50844, Penumbra

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB8405

Provider

Novus Biologicals

Product name

Mouse Monoclonal TSPAN33 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects HEK293 human embryonic kidney cell line transfected with human TSPAN33 by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

The Neonatal and Adult Human Testis Defined at the Single-Cell Level.

Sohni A, Tan K, Song HW, Burow D, de Rooij DG, Laurent L, Hsieh TC, Rabah R, Hammoud SS, Vicini E, Wilkinson MF
Cell reports 2019 Feb 5;26(6):1501-1517.e4

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of TSPAN33 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated overnight with Recombinant Human IL-4 (Catalog # 204-IL) and Recombinant Human CD40 Ligand/TNFSF5 (Catalog # 6245-CL) were stained with Mouse Anti-Human TSPAN33 Monoclonal Antibody (Catalog # MAB8405) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).