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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-40755 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FXR Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: AECLLTEIQC KSKRLRKNVK QHADQTVNED SEGRDLRQVT STTKSCREKT
- Concentration
- 0.5 mg/mL
Submitted references Effect of Gegen Qinlian Decoction on Hepatic Gluconeogenesis in ZDF Rats with Type 2 Diabetes Mellitus Based on the Farnesol X Receptor/Ceramide Signaling Pathway Regulating Mitochondrial Metabolism and Endoplasmic Reticulum Stress.
Ileo-colonic delivery of conjugated bile acids improves glucose homeostasis via colonic GLP-1-producing enteroendocrine cells in human obesity and diabetes.
Zhou Q, Song N, Wang SQ, Wang Y, Zhao YK, Zhu XD
Evidence-based complementary and alternative medicine : eCAM 2021;2021:9922292
Evidence-based complementary and alternative medicine : eCAM 2021;2021:9922292
Ileo-colonic delivery of conjugated bile acids improves glucose homeostasis via colonic GLP-1-producing enteroendocrine cells in human obesity and diabetes.
Calderon G, McRae A, Rievaj J, Davis J, Zandvakili I, Linker-Nord S, Burton D, Roberts G, Reimann F, Gedulin B, Vella A, LaRusso NF, Camilleri M, Gribble FM, Acosta A
EBioMedicine 2020 May;55:102759
EBioMedicine 2020 May;55:102759
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse glomerular endothelial cell lysate using an anti-Farnesoid X Receptor polyclonal antibody (Product # PA5-40755). Primary Antibody Dilution: 1:1000; Secondary Antibody: Anti-rabbit-HRP. LANE 1) 50µg mouse glomerular endothelial lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human lung cells using an anti-Farnesoid X Receptor polyclonal antibody (Product # PA5-40755).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-FXR Polyclonal Antibody (Product # PA5-40755) and a ~48 kDa band corresponding to FXR was observed across the cell lines tested. Uncharacterized band (*) was observed at ~60KDa in THP-1 cell lysate. Whole cell extracts (30 µg) of HepG2 (Lane 1), BEWO (Lane 2), HeLa (Lane 3), THP-1 (Lane 4), A549 (Lane 5) and Jurkat (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of human kidney tissue using an anti-Farnesoid X Receptor polyclonal antibody (Product # PA5-40755).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Effects of the treatment of Gegen Qinlian Decoction (GQD) for 12 weeks on FXR, Sptlc2, and Smpd3 expressions in the intestinal tissue of ZDF (fa/fa) rats. The levels of FXR, Sptlc2, and Smpd3 proteins were detected by the immunohistochemistry assay ((a), (b)) and western blot assay ((c), (d)). The levels of FXR, Sptlc2, and Smpd3 mRNAs were detected by the qRT-PCR assay (e). Results were presented as mean +- SD ( n = 8). ** P < 0.01 versus the blank group; ## P < 0.01 versus the model group (magnification x200).
