18712-1-AP

antibody from Proteintech Group

Targeting: CKM CKMM

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

18712-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#18712-1-AP, RRID:AB_2260650

Product name

CKM-Specific antibody

Antibody type

Polyclonal

Description

CKM-Specific antibody (Cat. #18712-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Oxaloacetate and Ketone Bodies Synergistically Promote Myoblast Differentiation in L6 Cells.

Onuki Y, Nanashima N, Sasaki Y, Kojima-Yuasa A, Norikura T
Molecules (Basel, Switzerland) 2025 May 9;30(10)

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Effects of transient receptor potential channel activation during myogenic progression and skeletal muscle function during voluntary exercise.

Yamamoto H, Kojima K, Kasai S, Takimoto Y, Kato A, Yamamoto K, Moriya Y, Koida A, Agata U
Bioscience, biotechnology, and biochemistry 2025 Dec 18;90(1):66-75

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Ligustilide covalently binds to Cys254 of the creatine kinase, M-type protein, ameliorating acute myocardial ischemia by enhancing the creatine phosphate level.

Zhang K, Luan G, Zhang J, Wang S, Jiang M, Bai G
Phytomedicine : international journal of phytotherapy and phytopharmacology 2025 Apr;139:156532

---

Pb inhibited C2C12 myoblast differentiation by regulating HDAC2.

Gu X, Shen N, Huang C, Wang HL
Toxicology 2023 Nov;499:153639

---

Ang-(1-7) protects skeletal muscle function in aged mice.

Li Y, Song J, Jiang Y, Yang X, Cao L, Xiao C, Li S, Dong B, Huang X
BMC musculoskeletal disorders 2021 Sep 21;22(1):809

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Naturally Occurring Mutations to Muscle-Type Creatine Kinase Impact Its Canonical and Pharmacological Activities in a Substrate-Dependent Manner In Vitro.

Mosher EP, Eberhard CD, Bumpus NN
Molecular pharmacology 2021 Dec;100(6):588-596

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SAR1B senses leucine levels to regulate mTORC1 signalling.

Chen J, Ou Y, Luo R, Wang J, Wang D, Guan J, Li Y, Xia P, Chen PR, Liu Y
Nature 2021 Aug;596(7871):281-284

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KLF3 promotes the 8-cell-like transcriptional state in pluripotent stem cells.

Hao J, Yang X, Zhang C, Zhang XT, Shi M, Wang SH, Mi L, Zhao YT, Cao H, Wang Y
Cell proliferation 2020 Nov;53(11):e12914

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

mouse heart tissue were subjected to SDS PAGE followed by western blot with 18712-1-AP(CKM-Specific antibody) at dilution of 1:2000

Sample type

tissue

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using CKM antibody 18712-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human skeletal muscle using 18712-1-AP(CKM-Specific antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human skeletal muscle using 18712-1-AP(CKM-Specific antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue