Antibody data
- Antibody Data
 - Antigen structure
 - References [0]
 - Comments [0]
 - Validations
 - Western blot [1]
 - Immunocytochemistry [1]
 - Immunoprecipitation [1]
 
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Validation data
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- Product number
 - H00001158-M06 - Provider product page

 - Provider
 - Abnova Corporation
 - Proper citation
 - Abnova Corporation Cat#H00001158-M06, RRID:AB_1574002
 - Product name
 - CKM monoclonal antibody (M06), clone 2F3-B11
 - Antibody type
 - Monoclonal
 - Description
 - Mouse monoclonal antibody raised against a full-length recombinant CKM.
 - Antigen sequence
 MPFGNTHNKFKLNYKPEEEYPDLSKHNNHMAKVLT
LELYKKLRDKETPSGFTVDDVIQTGVDNPGHPFIM
TVGCVAGDEESYEVFKELFDPIISDCHGGYKPTDK
HKTDLNHENLKGGDDLDPNYVLSSRVRTGRSIKGY
TLPPHCSRGERRAVEKLSVEALNSLAGEFKGKYYP
LKSMTEKEQQQLIDDHFLFDKPVSPLLLASGMARD
WPDARGIWHNDNKSLLVWVNEEDHLRVISMEKGGN
MKEVFRRFCVGLQKIEEIFKKAGHPFMWNQHLGYV
LTCPSNLGTGLRGGVHVKLAHLSKHPKFEEILTRL
RLQKRGTGGVDTAAVGSVFDVSNADRLGSSEVEQV
QLVVDGVKLMVEMEKKLEKGQSIDDMIPAQK- Isotype
 - IgG
 - Antibody clone number
 - 2F3-B11
 - Storage
 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
 
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					Supportive validation
					
									
				
				- Submitted by
 - Abnova Corporation (provider)
 - Main image
 
- Experimental details
 - Western Blot analysis of CKM expression in transfected 293T cell line by CKM monoclonal antibody (M06), clone 2F3-B11.Lane 1: CKM transfected lysate (Predicted MW: 43.1 KDa).Lane 2: Non-transfected lysate.
 
							
					Supportive validation
					
									
				
				- Submitted by
 - Abnova Corporation (provider)
 - Main image
 
- Experimental details
 - Immunofluorescence of monoclonal antibody to CKM on HeLa cell . [antibody concentration 40 ug/ml]
 - Validation comment
 - Immunofluorescence
 - Protocol
 - Protocol
 
							
					Supportive validation
					
									
				
		- Submitted by
 - Abnova Corporation (provider)
 - Main image
 
- Experimental details
 - Immunoprecipitation of CKM transfected lysate using anti-CKM monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with CKM MaxPab rabbit polyclonal antibody.
 - Validation comment
 - Immunoprecipitation
 - Protocol
 - Protocol