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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-28920 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Creatine Kinase MM Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: 293T, A431, HeLaS3. Predicted reactivity: Mouse (97%), Rat (97%), Xenopus laevis (88%), Dog (97%), Pig (99%), Rabbit (98%), Chicken (91%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Creatine Kinase MB Polyclonal Antibody detects Creatine kinase (muscle) protein at cytosol on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. Creatine Kinase MB Polyclonal Antibody (Product # PA5-28920) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 (A-E) Blood clot aspirates from the left side of the pelvis ( A , hematoxylin-eosin; B , INSM1; C , CD56; D , troponin-T; E , CK-MB). (F-H) Primary lesion in the ethmoid sinus ( F , hematoxylin-eosin; G , troponin-T; H , CK-MB). x200. Deparaffinized slide sections were heated to retrieve the antigen (citrate, pH 6.0 for CD56, Troponin-T, and CK-MB; ethylenediaminetetraacetic acid, pH 9.0 for INSM1). They were immersed in hydrogen peroxide to block the endogenous peroxidase activity. The slide sections were incubated with the following primary monoclonal antibodies; INSM1 (0.67 mug/mL, clone A-8, Santa Cruz Biotechnology, Inc.), CD56 (0.11 mug/mL, clone 1B6, Leica Biosystems Newcastle Ltd), cardiac troponin T (0.5 mug/mL, clone 13-11, ThermoFisher Scientific), and polyclonal antibody for CK-MB (2.5 mug/mL, ThermoFisher Scientific, catalog number PA5-28920). Then, the slide sections were incubated with horseradish peroxidase-polymer secondary antibody at room temperature. They were visualized with 0.05% 3, 3'-diaminobenzidine containing hydrogen peroxide, and counterstained with hematoxylin. The slides were scanned under the Olympus BX53F microscope (Olympus, Tokyo, Japan). Negative (IgG) and positive (human heart) slides were prepared for troponin-T and CK-MB staining (data not shown).
