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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-C289958 - Provider product page
- Provider
- LSBio
- Product name
- Anti-NTMT1 / C9orf32 Antibody (aa1-50) LS-C289958
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Horse, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100µl
- Storage
- Store at 2-8°C for up to 1 year.
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Supportive validation
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- LSBio (provider)
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- Experimental details
- Detection of Human and Mouse NRMT by Western Blot (h and m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), Ramos (R; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NRMT antibody used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. NRMT was also immunoprecipitated by rabbit anti-NRMT antibody BL11696, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
