AF7159

antibody from R&D Systems

Targeting: NTMT1 AD-003, C9orf32, HOMT1A, METTL11A, NRMT, NRMT1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF7159

Provider

R&D Systems

Product name

Human alpha-N-terminal Methyltransferase 1A/METTL11A Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human alpha-N-terminal Methyltransferase 1A/METTL11A in direct ELISAs and Western blots.

Reactivity

Human

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

Q9BV86


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human alpha-N-terminal Methyltransferase 1A/ METTL11A by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human alpha-N-terminal Methyltransferase 1A/METTL11A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7159) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for alpha-N-terminal Methyltransferase 1A/ METTL11A at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.