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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- MA5-18290 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CALCOCO2 Monoclonal Antibody (P17410)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Jurkat, Raji, NCI-H929.
- Antibody clone number
- P17410
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CALCOCO2 Monoclonal Antibody (P17410) detects NDP52 protein by western blot analysis. A. 30 µg Jurkat whole cell lysate/extract. B. 30 µg Raji whole cell lysate/extract. C. 30 µg NCI-H929 whole cell lysate/extract.10 % SDS-PAGE. CALCOCO2 Monoclonal Antibody (P17410) (Product # MA5-18290) dilution: 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-CALCOCO2 Monoclonal Antibody (P17410) (Product # MA5-18290) and a 55kDa band corresponding to CALCOCO2 was observed across the cell lines tested and tissues tested with uncharacterized bands (*). Whole cell extracts (30 µg lysate) of HeLa (Lane 1), HeLa (Lane 2), Mouse Testis (Lane 3), Mouse Heart (Lane 4) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
