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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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- Product number
- PA5-62735 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AdoHcyase 3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: GKVPQASAMK RSDPHHQHQR HRDGGEALVS PDGTVTEAPR TVKKQIQFAD QKQEFNKRPT KI Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 98%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.3 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of AdoHcyase 3 in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using an AdoHcyase 3 Polyclonal Antibody (Product # PA5-62735).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of AdoHcyase 3 in human cell line PC-3 shows positivity in cytoplasm & nucleus but excluded from the nucleoli. Samples were probed using an AdoHcyase 3 Polyclonal Antibody (Product # PA5-62735).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of AdoHcyase 3 in human stomach and liver tissues using AdoHcyase 3 Polyclonal Antibody (Product # PA5-62735). Corresponding AHCYL2 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of AdoHcyase 3 in human small intestine using AdoHcyase 3 Polyclonal Antibody (Product # PA5-62735) shows weak cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of AdoHcyase 3 in human cerebral cortex using AdoHcyase 3 Polyclonal Antibody (Product # PA5-62735) shows weak to moderate cytoplasmic positivity in neurons.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of AdoHcyase 3 in human liver using AdoHcyase 3 Polyclonal Antibody (Product # PA5-62735) shows no positivity in hepatocytes as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of AdoHcyase 3 in human stomach using AdoHcyase 3 Polyclonal Antibody (Product # PA5-62735) shows moderate to strong membranous positivity in parietal cells.
