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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-22214 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AdoHcyase 3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, Raji. Predicted reactivity: Mouse (100%), Rat (99%), Zebrafish (96%), Xenopus laevis (99%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.66 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using AdoHcyase 3 Polyclonal Antibody (Product # PA5-22214). Sample (30 µg of whole cell lysate). Lane A: Raji . 7.5% SDS PAGE. AdoHcyase 3 Polyclonal Antibody (Product # PA5-22214) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- AHCYL2 antibody [N3C3] detects AHCYL2 protein at cytoplasm by immunofluorescent analysis. Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AHCYL2 protein stained by AHCYL2 antibody [N3C3] (Product # PA5-22214) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of AdoHcyase 3 was performed in paraffin-embedded human rectal carcinoma tissue using AdoHcyase 3 Polyclonal Antibody (Product # PA5-22214) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using AHCYL2 (Product # PA5-22214) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
