Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [10]
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Validation data
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- Product number
- NBP1-81322 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-81322, RRID:AB_11006720
- Product name
- Rabbit Polyclonal GRHL1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human GRHL1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Evidence for multiple roles for grainyhead-like 2 in the establishment and maintenance of human mucociliary airway epithelium.[corrected].
Gao X, Vockley CM, Pauli F, Newberry KM, Xue Y, Randell SH, Reddy TE, Hogan BL
Proceedings of the National Academy of Sciences of the United States of America 2013 Jun 4;110(23):9356-61
Proceedings of the National Academy of Sciences of the United States of America 2013 Jun 4;110(23):9356-61
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GRHL1 Antibody [NBP1-81322] - Analysis in control (vector only transfected HEK293T lysate) and GRHL1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81322] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81322] - Staining of human tonsil shows moderate nuclear positivity in squamous epithelial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81322] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81322] - Staining of human skin shows moderate to strong nuclear positivity in a subset of squamous epithelial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81322] - Staining of human esophagus shows moderate to strong nuclear positivity in squamous epithelial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81322] - Staining of human esophagus, placenta, skeletal muscle and skin using Anti-GRHL1 antibody NBP1-81322 (A) shows similar protein distribution across tissues to independent antibody NBP1-81321 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81322] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81322] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81322] - Staining of human skin shows moderate to strong nuclear positivity in squamous epithelial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81322] - Staining in human skin and skeletal muscle tissues using NBP1-81322 antibody. Corresponding GRHL1 RNA-seq data are presented for the same tissues.