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Western blot
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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- HPA006420 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA006420, RRID:AB_1079025
- Product name
- Anti-GRHL1
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human GRHL1, Gene description: grainyhead-like 1 (Drosophila), Alternative Gene Names: LBP-32, MGR, TFCP2L2, Validated applications: WB, IHC, ICC, Uniprot ID: Q9NZI5, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Evidence for multiple roles for grainyhead-like 2 in the establishment and maintenance of human mucociliary airway epithelium
Gao X, Vockley C, Pauli F, Newberry K, Xue Y, Randell S, Reddy T, Hogan B
Proceedings of the National Academy of Sciences 2013;110(23):9356-9361
Proceedings of the National Academy of Sciences 2013;110(23):9356-9361
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and GRHL1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402346).
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human skin and skeletal muscle tissues using HPA006420 antibody. Corresponding GRHL1 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
