HPA006421

EMX1 antibody from Atlas Antibodies

Eligible for validation within the Antibodypedia Validation Initiative

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Validation data

Product number: HPA006421
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA006421, RRID:AB_888483
Product name: Anti-EMX1
Provider product page: Atlas Antibodies - HPA006421
Antibody type: Polyclonal
Description: Affinity purified using the PrEST antigen as affinity ligand
Reactivity: Human, Mouse
Host: Rabbit
Conjugate: Unconjugated
Antigen sequence: RSLYGGPELVFPEAMNHPALTVHPAHQLGASPLQP PHSFFGAQHRDPLHFYPWVLRNRFFGHRFQASDVP QDGLLLHGPFARKPKRI
Isotype: IgG
Vial size: 100 µl
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Provider	Type	Product Number
- No reagents -

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells.

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of mouse E11 embryonic brain shows moderate nuclear positivity in the developing cortical plate.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human kidney shows moderate nuclear positivity in cells in a subset of renal tubules.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.
Sample type: HUMAN

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
Main image
Experimental details: Immunohistochemistry analysis in human kidney and skeletal muscle tissues using HPA006421 antibody. Corresponding EMX1 RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.