Explore
Validate
LearnPA5-51036
antibody from Invitrogen Antibodies
Targeting: PTGES
MGST-IV, MGST1-L1, MGST1L1, PIG12, TP53I12
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-51036 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PTGES Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PTGES Polyclonal Antibody detects endogenous levels of PTGES protein.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Small Molecule Interactome Mapping by Photo-Affinity Labeling (SIM-PAL) to Identify Binding Sites of Small Molecules on a Proteome-Wide Scale.
Flaxman HA, Miyamoto DK, Woo CM
Current protocols in chemical biology 2019 Dec;11(4):e75
Current protocols in chemical biology 2019 Dec;11(4):e75
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PTGES in A549 cells. Samples were probed using PTGES Polyclonal Antibody (Product # PA5-51036).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-PTGES Polyclonal Antibody(Product # PA5-51036) and a 18kDa band corresponding to PTGES was observed across positive cell lines tested. Membrane enriched extracts (30 µg lysate) of A-431 (Lane 1), DU 145 (Lane 2) and BeWo (Lane 3) (positive cell lines); and HEL 92.1.7 (Lane 4), LNCaP (Lane 5) and PC-3 (Lane 6) (negative cell lines) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
