Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 91370ACTMOTIF - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- JMJD2D/KDM4D Recombinant Mouse Monoclonal Antibody, AbFlex®
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 10 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- AbFlex JMJD2D/KDM4D antibody tested by Western blot. 20 µg of HEK293 nuclear extract was run on SDS-PAGE and probed with antibody at 0.5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- AbFlex JMJD2D/KDM4D antibody tested by Dot blot. Recombinant KDM proteins were spotted onto nitrocellulose membrane (nanograms indicated on row labels) and probed with antibody at 1 µg/mL. Col. 1: KDM4A. Col. 2: KDM4B. Col. 3: KDM4C. Col. 4: KDM5A. Col. 5: KDM5B. Col. 6: KDM5C.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- AbFlex JMJD2D/KDM4D antibody tested by Immunoprecipitation. 4 µg of AbFlex JMJD2D/KDM4 antibody (Lane1) or isotype control mouse IgG (Lane 2) was used to immunoprecipitate JMJD2D/KDM4D from 500 µg of HEK293 extract, which was then run on SDS-PAGE and probed with the same antibody at 2 µg/mL. The appearance of antibody light chain at 25 kDa is observed as a cross-reactivity between the WB / IP antibody, and is common.
