Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-03357 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-03357, RRID:AB_1521485
- Product name
- Rabbit Polyclonal JMJD2D Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references JMJD2 promotes acquired cisplatin resistance in non-small cell lung carcinoma cells.
Histone demethylase JMJD2C is a coactivator for hypoxia-inducible factor 1 that is required for breast cancer progression.
Duan L, Perez RE, Chastain PD 2nd, Mathew MT, Bijukumar DR, Maki CG
Oncogene 2019 Jul;38(28):5643-5657
Oncogene 2019 Jul;38(28):5643-5657
Histone demethylase JMJD2C is a coactivator for hypoxia-inducible factor 1 that is required for breast cancer progression.
Luo W, Chang R, Zhong J, Pandey A, Semenza GL
Proceedings of the National Academy of Sciences of the United States of America 2012 Dec 4;109(49):E3367-76
Proceedings of the National Academy of Sciences of the United States of America 2012 Dec 4;109(49):E3367-76
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: JMJD2D Antibody [NBP1-03357] - Western Blot Image of anti-JMJD2D. Whole cell protein from PC3 (lane 1), SHSY5Y (lane 2), and HeLa (lane 3) was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-JMJD2D in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
