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Western blot
Immunocytochemistry
Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- HPA029329 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA029329, RRID:AB_10601240
- Product name
- Anti-NRF1
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human NRF1, Gene description: nuclear respiratory factor 1, Alternative Gene Names: ALPHA-PAL, EWG, Validated applications: ChIP, ICC, IHC, WB, Uniprot ID: Q16656, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Mitochondria “fuel” breast cancer metabolism: Fifteen markers of mitochondrial biogenesis label epithelial cancer cells, but are excluded from adjacent stromal cells
Sotgia F, Whitaker-Menezes D, Martinez-Outschoorn U, Salem A, Tsirigos A, Lamb R, Sneddon S, Hulit J, Howell A, Lisanti M
Cell Cycle 2014;11(23):4390-4401
Cell Cycle 2014;11(23):4390-4401
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-NRF1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- ChIP-Exo-Seq composite graph for Anti-NRF1 (HPA029329, Lot A118486) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.
