GTX103179

antibody from GeneTex

Targeting: NRF1 ALPHA-PAL, EWG

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX103179

Provider

GeneTex

Proper citation

GeneTex Cat#GTX103179, RRID:AB_11168915

Product name

NRF1 antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

References

Submitted references

1,25-Dihydroxyvitamin D₃ modulates the effects of sublethal BPA on mitochondrial function via activating PI3K-Akt pathway and 17β-estradiol secretion in rat granulosa cells.

Lee CT, Wang JY, Chou KY, Hsu MI
The Journal of steroid biochemistry and molecular biology 2019 Jan;185:200-211

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A mitohormetic response to pro-oxidant exposure in the house mouse.

Zhang Y, Humes F, Almond G, Kavazis AN, Hood WR
American journal of physiology. Regulatory, integrative and comparative physiology 2018 Jan 1;314(1):R122-R134

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Caveolin-1 Expression Ameliorates Nephrotic Damage in a Rabbit Model of Cholesterol-Induced Hypercholesterolemia.

Chen YH, Lin WW, Liu CS, Hsu LS, Lin YM, Su SL
PloS one 2016;11(4):e0154210

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Rosiglitazone activation of PPARγ-dependent signaling is neuroprotective in mutant huntingtin expressing cells.

Chiang MC, Cheng YC, Nicol CJ, Lin KH, Yen CH, Chen SJ, Huang RN
Experimental cell research 2015 Nov 1;338(2):183-93

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Caveolin-1 provides palliation for adverse hepatic reactions in hypercholesterolemic rabbits.

Chen YH, Lin WW, Liu CS, Hsu LS, Lin YM, Su SL
PloS one 2014;9(1):e71862

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Physiological cyclic strain promotes endothelial cell survival via the induction of heme oxygenase-1.

Liu XM, Peyton KJ, Durante W
American journal of physiology. Heart and circulatory physiology 2013 Jun 15;304(12):H1634-43

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 10% SDS PAGE GTX103179 diluted at 1:1000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

NRF1 antibody detects NRF1 protein by western blot analysis.A. 30 £gg 293T whole cell lysate/extract B. 30 £gg 293T nuclear lysate/extract C. 30 £gg A431 whole cell lysate/extract | D. 30 £gg A431 nuclear lysate/extract E. 30 £gg HeLa whole cell lysate/extract F. 30 £gg HeLa nuclear lysate/extract G. 30 £gg HepG2 whole cell lysate/extract H. 30 £gg HepG2 nuclear lysate/extract10 % SDS-PAGENRF1 antibody (GTX103179) dilution: 1:1000

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

NRF1 antibody detects NRF1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NRF1 protein stained by NRF1 antibody (GTX103179) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

NRF1 antibody immunoprecipitates NRF1 protein in IP experiments.IP samples: 293T whole cell extractA. Control with 4 £gg of preimmune Rabbit IgGB. Immunoprecipitation of NRF1 protein by 4 £gg NRF1 antibody (GTX103179)10 % SDS-PAGEThe immunoprecipitated NRF1 protein was detected by NRF1 antibody (GTX103179) diluted at 1:1000.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

NRF1 antibody detects NRF1 protein at nucleus on human lung adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung adenocarcinoma. NRF1 antibody (GTX103179) diluted at 1:250.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

NRF1 antibody detects NRF1 protein at nucleus on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. NRF1 antibody (GTX103179) dilution: 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

NRF1 antibody detects NRF1 protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. NRF1 antibody (GTX103179) dilution: 1:500.