H00004899-M01-50ug

antibody from Novus Biologicals

Targeting: NRF1 ALPHA-PAL, EWG

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

H00004899-M01-50ug

Provider

Novus Biologicals

Product name

Mouse Monoclonal Nrf1 Antibody

Antibody type

Monoclonal

Description

IgG purified. NRF1 - nuclear respiratory factor 1

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Vial size

50 ug

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Nrf1 Antibody (2F9) [H00004899-M01] - Analysis of NRF1 over-expressed 293 cell line, cotransfected with NRF1 Validated Chimera RNAi ( Cat # H00004899-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NRF1 monoclonal antibody (M01), clone 2F9 (Cat # H00004899-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Nrf1 Antibody (2F9) [H00004899-M01] - Analysis of NRF1 expression in transfected 293T cell line by NRF1 monoclonal antibody (M01), clone 2F9.Lane 1: NRF1 transfected lysate (Predicted MW: 55.7 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Nrf1 Antibody (2F9) [H00004899-M01] - NRF1 monoclonal antibody (M01), clone 2F9. Analysis of NRF1 expression in human Skeletal muscle.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Nrf1 Antibody (2F9) [H00004899-M01] - NRF1 monoclonal antibody (M01), clone 2F9. Analysis of NRF1 expression in HeLa.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: Nrf1 Antibody (2F9) [H00004899-M01] - Detection limit for recombinant GST tagged NRF1 is approximately 0.03ng/ml as a capture antibody.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Nrf1 Antibody (2F9) [H00004899-M01] - Analysis of monoclonal antibody to NRF1 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.