Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [3]
- Immunohistochemistry [4]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- MA5-32782 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NRF1 Recombinant Rabbit Monoclonal Antibody (JM89-63)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JM89-63
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Alcalase Potato Protein Hydrolysate-PPH902 Enhances Myogenic Differentiation and Enhances Skeletal Muscle Protein Synthesis under High Glucose Condition in C2C12 Cells.
Chen YJ, Chang CF, Angayarkanni J, Lin WT
Molecules (Basel, Switzerland) 2021 Oct 30;26(21)
Molecules (Basel, Switzerland) 2021 Oct 30;26(21)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NRF1 in Hela cell (1) and mouse heart tissue (2) lysate using a NRF1 Monoclonal antibody (Product # MA5-32782) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of NRF1 was achieved by transfecting HeLa cells with NRF1 specific siRNAs (Silencer® select Product # s32459, s32460). Western blot analysis (Fig. a) was performed using Whole cell extracts from the NRF1 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with NRF1 Polyclonal Antibody (Product # MA5-32782, 1:500 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to NRF1..
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti- NRF1 Monoclonal Antibody (Product # MA5-32782) and a 60 kDa band corresponding to NRF1 was observed across cell lines tested. Modified Whole cell extracts (1%SDS) (30 µg lysate) of HeLa (Lane 1), PC-3 (Lane 2), A-431 (Lane 3), U-2 OS (Lane 4) and A549 (Lane 5) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of NRF1 in Hela cells using a NRF1 Monoclonal antibody (Product # MA5-32782) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of NRF1 in MCF-7 cells using a NRF1 Monoclonal antibody (Product # MA5-32782) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of NRF1 in SH-SY5Y cells using a NRF1 Monoclonal antibody (Product # MA5-32782) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NRF1 of paraffin-embedded rat brain tissue using a NRF1 Monoclonal antibody (Product #MA5-32782). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NRF1 of paraffin-embedded Human tonsil tissue using a NRF1 Monoclonal antibody (Product #MA5-32782). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NRF1 of paraffin-embedded Human thyroid tissue using a NRF1 Monoclonal antibody (Product #MA5-32782). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NRF1 of paraffin-embedded Mouse colon tissue using a NRF1 Monoclonal antibody (Product #MA5-32782). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of NRF1 in 293T cells using a NRF1 Monoclonal Antibody (Product # MA5-32782) at a dilution of 1:100, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 9 Effect of PPH902 on the activation of mitochondrial biogenesis: Representative Western blotting shows changes in the levels of NRF1 and TFAM in C2C12 cells. *** p < 0.001 indicates a significant difference with respect to control groups and ## p < 0.01 and ### p < 0.001 indicates a significance difference with respect to the high glucose challenge groups.