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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB10295 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10295, RRID:AB_1676647
- Product name
- Nrf1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against partial recombinant Nrf1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Mitochondrial benzodiazepine receptors regulate oxygen homeostasis in the early mouse embryo.
c-MYC apoptotic function is mediated by NRF-1 target genes.
Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR, Stahl E, Bolouri MS, Ray HN, Sihag S, Kamal M, Patterson N, Lander ES, Mann M
Cell 2003 Nov 26;115(5):629-40
Cell 2003 Nov 26;115(5):629-40
Mitochondrial benzodiazepine receptors regulate oxygen homeostasis in the early mouse embryo.
O'Hara MF, Nibbio BJ, Craig RC, Nemeth KR, Charlap JH, Knudsen TB
Reproductive toxicology (Elmsford, N.Y.) 2003 Jul-Aug;17(4):365-75
Reproductive toxicology (Elmsford, N.Y.) 2003 Jul-Aug;17(4):365-75
c-MYC apoptotic function is mediated by NRF-1 target genes.
Morrish F, Giedt C, Hockenbery D
Genes & development 2003 Jan 15;17(2):240-55
Genes & development 2003 Jan 15;17(2):240-55
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using Nrf1 polyclonal antibody (Cat # PAB10295) shows detection of a 67 KDa band corresponding to human Nrf1 in a (Lane 1) HeLa nuclear extract and (Lane 2) whole cell lysate (molecular weight marker not shown).Approximately 10 ug of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose.The blot was incubated with a 1 : 500 dilution of the antibody at room temperature for 1 h followed by detection using IRDye™700 labeled Goat-a-Rabbit IgG [H&L] diluted 1 : 2,500.IRDye™700 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is atrademark of LI-COR, Inc.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemistry of Nrf1 polyclonal antibody (Cat # PAB10295) was used at a 5 ug/mL to detect NRF1 in a variety of tissues.This image shows Nrf1 staining of human testis.The antibody shows nuclear staining in lymphocytes.Tissue was formalin-fixed andparaffin embedded.Personal Communication, Tina Roush,Life Span Biosciences, Seattle, WA.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
