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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [2]
- Other assay [1]
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- Product number
- PA5-27854 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NRF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, 293T nuclear, A431, A431 nuclear, HeLa, HeLa nuclear, HepG2, HepG2 nuclear. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (94%), Xenopus laevis (94%), Chicken (99%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.32 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of NRF1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: NRF1 Polyclonal Antibody (Product # PA5-27854) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NRF1 was performed using HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with NRF1 Rabbit Polyclonal Antibody (Product # PA5-27854) at 1:250 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the composite image showing nuclear localization of NRF1. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NRF1 Polyclonal Antibody immunoprecipitates NRF1 protein in IP experiments. IP samples: 293T whole cell extract. A. Control with 4 µg of preimmune Rabbit IgG. B. Immunoprecipitation of NRF1 protein by 4 µg NRF1 Polyclonal Antibody (Product # PA5-27854). 10 % SDS-PAGE. The immunoprecipitated NRF1 protein was detected by NRF1 Polyclonal Antibody (Product # PA5-27854) diluted at 1:1,000.
