AP09128PU-N

antibody from Acris Antibodies GmbH

Targeting: NRF1 ALPHA-PAL, EWG

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP09128PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP09128PU-N, RRID:AB_2035805

Product name

anti NRF1 (1-534)

Antibody type

Polyclonal

Antigen

Recombinant Mouse NRF1 protein corresponding to aa 1-534 of the native protein.

Reactivity

Human, Mouse, Rat, Chicken/Avian, Zebrafish

Host

Rabbit

Isotype

IgG

Vial size

0.5 mg

Concentration

2.3 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using Protein A Purified AP09128PU-N NRF1 antibody shows detection of a 67-kDa band corresponding to Human NRF1 in a (Lane 1) HeLa nuclear extract and (Lane 2) whole cell lysate (molecular weight marker not shown). ~10 µg of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1/500 dilution of the antibody at RT for 1 h followed by detection using IRDye™700 labeled Goat anti-Rabbit IgG [H&L] diluted 1/2,500. IRDye™700 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry. AP09128PU-N NRF1 antibody staining of Formalin-Fixed, Paraffin Embedded Human testis. This Affinity purified antibody was used at 5 µg/ml to detect NRF1 in a variety of tissues. It shows nuclear staining in lymphocytes.Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.