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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00029079-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00029079-M01, RRID:AB_1136958
- Product name
- MED4 monoclonal antibody (M01), clone 2B10
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant MED4.
- Antigen sequence
MAASSSGEKEKERLGGGLGVAGGNSTRERLLSALE
DLEVLSRELIEMLAISRNQKLLQAGEENQVLELLI
HRDGEFQELMKLALNQGKIHHEMQVLEKEVEKRDG
DIQQLQKQLKEAEQILATAVYQAKEKLKSIEKARK
GAISSEEIIKYAHRISASNAVCAPLTWVPGDPRRP
YPTDLEMRSGLLGQMNNPSTNGVNGHLPGDALAAG
RLPDVLAPQYPWQSNDMSMNMLPPNHSSDFLLEPP
GHNKEDEDDVEIMSTDSSSSSSESD- Isotype
- IgG
- Antibody clone number
- 2B10
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of MED4 expression in transfected 293T cell line by MED4 monoclonal antibody (M01), clone 2B10.Lane 1: MED4 transfected lysate(29.7 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged MED4 is 1 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to MED4 on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
