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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- R33853 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- ZBP-1 Antibody / IGF2BP1
- Antibody type
- Polyclonal
- Description
- This highly specific ZBP-1 antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/Flow cytometry/ELISA applications with human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
- Storage
- Aliquot and store the ZBP-1 antibody at -20oC.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of K562 lysate with ZBP1 antibody at 0.3ug/ml. Predicted molecular weight: ~64kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of A) human Caco-2, B) human K562, C) human HepG2 and D) mouse NIH-3T3 cell lysate with ZBP-1 antibody. Predicted molecular weight: ~64 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IF/ICC staining of fixed and permeabilized human HepG2 cells with ZBP-1 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IF/ICC staining of fixed and permeabilized mouse NIH-3T3 cells with ZBP-1 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human breast tissue with ZBP-1 antibody. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- FACS testing of fixed and permeabilized human HepG2 cells with ZBP-1 antibody (blue) at 10ug/ml and naive goat Ig (black).
