PA5-41820

antibody from Invitrogen Antibodies

Targeting: IGF2BP1 IMP-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-41820

Provider

Invitrogen Antibodies

Product name

IGF2BP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide sequence: PDEQIAQGPE NGRRGGFGSR GQPRQGSPVA AGAPAKQQQV DIPLRLLVPT Sequence homology: Cow: 93%; Dog: 100%; Guinea Pig: 93%; Horse: 93%; Human: 100%; Mouse: 93%; Pig: 100%; Rabbit: 100%; Rat: 100%; Sheep: 93%

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human HepG2 cell lysate using an anti-IGF2BP1 polyclonal antibody (Product # PA5-41820).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-IGF2BP1 Polyclonal Antibody (Product # PA5-41820) and 62 kDa band along with a higher molecular weight at ~ 120 kDa (probable glycosylated form) corresponding to IGF2BP1 was observed across all the cell lines tested except SK-OV-3. Whole cell extracts (30 µg lysate) of IMR-32 (Lane 1), PANC-1 (Lane 2), SK-OV-3 (Lane 3) and Hep G2 (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of IGF2BP1 was performed using 70% confluent log phase PANC-1 and SK-OV-3 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with IGF2BP1 Polyclonal Antibody (Product # PA5-41820) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000 dilution) for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear and cytoplasmic localization. Panel e represents SK-OV-3 cells having no expression of IG2BP1. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of human lung adenocarcinoma tissue using an anti-IGF2BP1 polyclonal antibody (Product # PA5-41820).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of human lung adenocarcinoma cells using an anti-IGF2BP1 polyclonal antibody (Product # PA5-41820). Primary Antibody Dilution:1:300; Secondary Antibody: Anti-rabbit-linker, Fbex-HRP.