F54545

antibody from NSJ Bioreagents

Targeting: IGF2BP1 IMP-1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54545

Provider

NSJ Bioreagents

Product name

IGF2BP1 Antibody / ZBP-1

Antibody type

Polyclonal

Description

This highly specific IGF2BP1 antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry applications with human and mouse samples.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the IGF2BP1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) HEK293, 2) RD, 3) Karpas 299, 4) Raji, 5) Jurkat, 6) HeLa, 7) HepG2 and 8) mouse NIH 3T3 cell lysate with IGF2BP1 antibody. Predicted molecular weight ~64 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human testis tissue with IGF2BP1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human lung adenocarcinoma tissue with IGF2BP1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized human HepG2 cells with IGF2BP1 antibody; Blue=isotype control, Green= IGF2BP1 antibody.