Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [3]
- Other assay [1]
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Validation data
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- Product number
- A302-355A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Pc2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Pc2 by western blot. Samples: Whole cell lysate (10 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Pc2 antibody (Product # A302-355A lot 2) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse Pc2 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3 and CT26 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Pc2 antibody (Product # A302-355A lot 2) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Pc2 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Pc2 antibody A302-355A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 10 µg/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Pc2 by immunohistochemistry. Sample: FFPE section of human bladder carcinoma. Antibody: Affinity purified rabbit anti- Pc2 (Product # A302-355A; lot 2) used at a dilution of 1:1,000 (1 µg/mL). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse Pc2 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- Pc2 (Product # A302-355A Lot 2) used at a dilution of 1:1,000 (1 µg/mL). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse Pc2 by immunohistochemistry. Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- Pc2 (Cat. No. A302-355A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Pc2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pc2 antibody (Product # A302-355A lot 2) used for IP at 6 µg per reaction. Pc2 was also immunoprecipitated by a previous lot of this antibody (A302-355A lot 1). For blotting immunoprecipitated Pc2 (Product # A302-355A) was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.