A302-355A

antibody from Invitrogen Antibodies

Targeting: CBX4 hPC2, NBP16, PC2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A302-355A

Provider

Invitrogen Antibodies

Product name

Pc2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human Pc2 by western blot. Samples: Whole cell lysate (10 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Pc2 antibody (Product # A302-355A lot 2) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse Pc2 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3 and CT26 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Pc2 antibody (Product # A302-355A lot 2) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human Pc2 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Pc2 antibody A302-355A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 10 µg/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human Pc2 by immunohistochemistry. Sample: FFPE section of human bladder carcinoma. Antibody: Affinity purified rabbit anti- Pc2 (Product # A302-355A; lot 2) used at a dilution of 1:1,000 (1 µg/mL). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse Pc2 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- Pc2 (Product # A302-355A Lot 2) used at a dilution of 1:1,000 (1 µg/mL). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse Pc2 by immunohistochemistry. Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- Pc2 (Cat. No. A302-355A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human Pc2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pc2 antibody (Product # A302-355A lot 2) used for IP at 6 µg per reaction. Pc2 was also immunoprecipitated by a previous lot of this antibody (A302-355A lot 1). For blotting immunoprecipitated Pc2 (Product # A302-355A) was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.