R1562P

antibody from Acris Antibodies GmbH

Targeting: CBX4 hPC2, NBP16, PC2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R1562P

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#R1562P, RRID:AB_1006163

Product name

anti CBX4 / PC2

Antibody type

Polyclonal

Antigen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 95-107 of Human PC2 protein.

Reactivity

Human, Mouse

Host

Rabbit

Vial size

0.1 mg

Concentration

0.8 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 2. Western blotting. Analysis shows the detection of human PC2 in probed lysates using Affinity Purified anti-hPC2 antibody. The panel on the left shows the blot probed with anti-hPC2. The panel on the right is the same blot reprobed with anti-FLAG antibody to confirm the presence of FLAG tagged recombinant PC2 in the lysate. In the left panel the band labeled as hPC2 is FLAG-tagged transfected hPC2 in 293T cells. The bands labeled with stars are likely endogenous hPC2. In the right panel the band labeled hPC2 is FLAG-tagged transfected hPC2 in 293T cells. Data contributed by Dr Ari Melnick, Albert Einstein College of Medicine.

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 1. Immunofluorescence Microscopy. Antibody anti-hPC2 was used for immunofluorescent imaging of human cells (U2OS). The image reveals the expected discrete nuclear structure that is termed the PcG body corresponding to the known localization of PC2 (see Satijn et al. below). IF was performed after fixation in PBS with 4% PF for 5 min, permeabilization with 0.5% Triton X100-PBS for 5 min, and blocking with 5% milk / 0.2% Tween for 1 h. Primary antibody used at 1:200 in 5% milk / 0.2% Tween for 1 h, secondary antibody for 30 min. All blocking and incubation steps carried out at 37°C. Nuclei were counterstained with Hoechst stain (blue). Data contributed by Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK.