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LearnMA5-17289
antibody from Invitrogen Antibodies
Targeting: POLR1G
A34.5, ASE-1, CAST, CD3EAP, PAF49, RPA34
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- MA5-17289 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PAF49 Monoclonal Antibody (GT1964)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, A375, A549, H1299, HCT116.
- Antibody clone number
- GT1964
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PAF49 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PAF49 Monoclonal Antibody (GT1964) (Product # MA5-17289) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PAF49 Monoclonal Antibody (GT1964) detects PPY protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg A431 whole cell lysate/extract. C. 30 µg HeLa whole cell lysate/extract. D. 30 µg HepG2 whole cell lysate/extract. E. 30 µg A375 whole cell lysate/extract.7.5 % SDS-PAGE. PAF49 Monoclonal Antibody (GT1964) (Product # MA5-17289) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CD3EAP antibody detects CD3EAP protein at Nucleus by immunofluorescent analysis. Sample: U2OS cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: CD3EAP protein stained by CD3EAP antibody (Product # MA5-17289) diluted at 1:500. Blue: Hoechst 33342 staining.
