A301-294A

antibody from Invitrogen Antibodies

Targeting: POLR1G A34.5, ASE-1, CAST, CD3EAP, PAF49, RPA34

Western blot (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A301-294A

Provider

Invitrogen Antibodies

Product name

PAF49 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human PAF49 by immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-PAF49 antibody A301-294A Lot2 used at a dilution of 1:5,000 (0.2 µg/ml). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human PAF49 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PAF49 antibody A301-294A (lot A301-294A-2) used for IP at 3 µg per reaction. PAF49 was also immunoprecipitated by rabbit anti-PAF49 antibody A301-295A. For blotting immunoprecipitated PAF49, A301-295A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.