Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- A301-283A-T - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- p66beta/GATAD2B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 10 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Detection of human p66beta/GATAD2B by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-p66beta/GATAD2B (Cat. No. A301-283A Lot2) used at a dilution of 1:1000 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human p66beta/GATAD2B by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-p66beta/GATAD2B antibody A301-283A (lot A301-283A-2) used for IP at 6 µg per reaction. p66beta/GATAD2B was also immunoprecipitated by rabbit anti-p66beta/GATAD2B antibody A301-283A-1 For blotting immunoprecipitated p66beta/GATAD2B, A301-282A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.