Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP1-90117 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-90117, RRID:AB_11014517
- Product name
- Rabbit Polyclonal TATA Element Modulatory Factor 1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human TATA Element Modulatory Factor 1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Membrane trafficking. The specificity of vesicle traffic to the Golgi is encoded in the golgin coiled-coil proteins.
Proteomic screen reveals Fbw7 as a modulator of the NF-κB pathway.
Loss of TMF/ARA160 protein renders colonic mucus refractory to bacterial colonization and diminishes intestinal susceptibility to acute colitis.
Wong M, Munro S
Science (New York, N.Y.) 2014 Oct 31;346(6209):1256898
Science (New York, N.Y.) 2014 Oct 31;346(6209):1256898
Proteomic screen reveals Fbw7 as a modulator of the NF-κB pathway.
Arabi A, Ullah K, Branca RM, Johansson J, Bandarra D, Haneklaus M, Fu J, Ariës I, Nilsson P, Den Boer ML, Pokrovskaja K, Grandér D, Xiao G, Rocha S, Lehtiö J, Sangfelt O
Nature communications 2012;3:976
Nature communications 2012;3:976
Loss of TMF/ARA160 protein renders colonic mucus refractory to bacterial colonization and diminishes intestinal susceptibility to acute colitis.
Bel S, Elkis Y, Lerer-Goldstein T, Nyska A, Shpungin S, Nir U
The Journal of biological chemistry 2012 Jul 20;287(30):25631-9
The Journal of biological chemistry 2012 Jul 20;287(30):25631-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TATA Element Modulatory Factor 1 Antibody [NBP1-90117] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-568
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TATA Element Modulatory Factor 1 Antibody [NBP1-90117] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TATA Element Modulatory Factor 1 Antibody [NBP1-90117] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TMF1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TATA Element Modulatory Factor 1 Antibody [NBP1-90117] - Staining of human gallbladder shows strong cytoplasmic positivity in glandular cells.