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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [1]
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- Product number
- HPA008729 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA008729, RRID:AB_1080310
- Product name
- Anti-TMF1
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human TMF1, Gene description: TATA element modulatory factor 1, Alternative Gene Names: ARA160, TMF, Validated applications: ICC, IHC, WB, Uniprot ID: P82094, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.2 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Dynamic movement of the Golgi unit and its glycosylation enzyme zones
ARFRP1 functions upstream of ARL1 and ARL5 to coordinate recruitment of distinct tethering factors to the trans-Golgi network.
The golgin coiled-coil proteins capture different types of transport carriers via distinct N-terminal motifs
The specificity of vesicle traffic to the Golgi is encoded in the golgin coiled-coil proteins
Proteomic screen reveals Fbw7 as a modulator of the NF-κB pathway
Loss of TMF/ARA160 Protein Renders Colonic Mucus Refractory to Bacterial Colonization and Diminishes Intestinal Susceptibility to Acute Colitis
Harada A, Kunii M, Kurokawa K, Sumi T, Kanda S, Zhang Y, Nadanaka S, Hirosawa K, Tokunaga K, Tojima T, Taniguchi M, Moriwaki K, Yoshimura S, Yamamoto-Hino M, Goto S, Katagiri T, Kume S, Hayashi-Nishino M, Nakano M, Miyoshi E, Suzuki K, Kitagawa H, Nakano A
Nature Communications 2024;15(1)
Nature Communications 2024;15(1)
ARFRP1 functions upstream of ARL1 and ARL5 to coordinate recruitment of distinct tethering factors to the trans-Golgi network.
Ishida M, Bonifacino JS
The Journal of cell biology 2019 Nov 4;218(11):3681-3696
The Journal of cell biology 2019 Nov 4;218(11):3681-3696
The golgin coiled-coil proteins capture different types of transport carriers via distinct N-terminal motifs
Wong M, Gillingham A, Munro S
BMC Biology 2017;15(1)
BMC Biology 2017;15(1)
The specificity of vesicle traffic to the Golgi is encoded in the golgin coiled-coil proteins
Wong M, Munro S
Science 2014;346(6209)
Science 2014;346(6209)
Proteomic screen reveals Fbw7 as a modulator of the NF-κB pathway
Arabi A, Ullah K, Branca R, Johansson J, Bandarra D, Haneklaus M, Fu J, Ariës I, Nilsson P, Den Boer M, Pokrovskaja K, Grandér D, Xiao G, Rocha S, Lehtiö J, Sangfelt O
Nature Communications 2012;3(1)
Nature Communications 2012;3(1)
Loss of TMF/ARA160 Protein Renders Colonic Mucus Refractory to Bacterial Colonization and Diminishes Intestinal Susceptibility to Acute Colitis
Bel S, Elkis Y, Lerer-Goldstein T, Nyska A, Shpungin S, Nir U
Journal of Biological Chemistry 2012;287(30):25631-25639
Journal of Biological Chemistry 2012;287(30):25631-25639
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TMF1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Sample type
- Human
- Protocol
- Protocol
