Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [2]
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Validation data
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- Product number
- MA5-18634 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Carboxypeptidase A1 Monoclonal Antibody (3F11)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Peptide Sequence: MRGLLVLSVL LGAVFGKEDF VGHQVLRISV ADEAQVQKVK ELEDLEHLQL DFWRGPAHPG SPIDVRVPFP SIQAVKIFLE SHGISYETMI EDVQSLLDEE QEQMFAFRSR ARSTDTFNYA TYHTLEEIYD FLDLLVAENP HLVSKIQIGN TYEGRPIYVL KFSTGGSKRP AIWIDTGIHS REWVTQASGV WFAKKITQDY GQDAAFTAIL DTLDIFLEIV TNPDGFAFTH STNRMWRKTR SHTAGSLCIG VDPNRNWDAG FGLSGASSNP CSETYRGKFA NSEVEVKSIV DFVKDHGNIK AFISIHSYSQ LLMYPYGYKT EPVPDQDELD QLSKAAVTAL ASLYGTKFNY GSIIKAIYQA SGSTIDWTYS QGIKYSFTFE LRDTGRYGFL LPASQIIPTA KETWLALLTI MEHTLNHPY
- Antibody clone number
- 3F11
- Concentration
- 0.2-1.0 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CPA1 expressed in transfected 293T cell line by anti-CPA1 monoclonal antibody (Product # MA5-18634), clone 3F11. Lane 1: CPA1 transfected lysate (47.1 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged CPA1 using anti-CPA1 monoclonal antibody (Product # MA5-18634) is approximately 0.3 ng/mL as a capture antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of CPA1 transfected lysate using anti-CPA1 monoclonal antibody (Product # MA5-18634) and Protein A Magnetic Beads, and immunoblotted with CPA1 rabbit polyclonal antibody.