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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunoprecipitation [4]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-C185626 - Provider product page
- Provider
- LSBio
- Product name
- LDB1 / CLIM2 Antibody (aa86-411) LS-C185626
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate). A: Raji. 10% SDS PAGE. CLIM2 / LDB1 antibody diluted at 1:1000.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate). A: Mouse brain. 10% SDS PAGE. CLIM2 / LDB1 antibody diluted at 1:1000.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunofluorescence of paraformaldehyde-fixed HeLa using LDB1 antibody at 1:200 dilution.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunofluorescence of paraformaldehyde-fixed HeLa using LDB1 antibody at 1:200 dilution.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- LDB1antibody immunoprecipitates LDB1 protein in IP experiments. IP Sample:1000 ug 293T whole cell lysate/extract. A. 20 ug 293T whole cell lysate/extract. B. Control with 2.5 ug of preimmune rabbit IgG. C. Immunoprecipitation of LDB1 protein by 2.5 ug of LDB1 antibody . 12% SDS-PAGE. The immunoprecipitated LDB1 protein was detected by LDB1antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- LDB1antibody immunoprecipitates LDB1 protein in IP experiments. IP Sample:1000 ug 293T whole cell lysate/extract. A. 20 ug 293T whole cell lysate/extract. B. Control with 2.5 ug of preimmune rabbit IgG. C. Immunoprecipitation of LDB1 protein by 2.5 ug of LDB1 antibody . 12% SDS-PAGE. The immunoprecipitated LDB1 protein was detected by LDB1antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- LDB1antibody immunoprecipitates LDB1 protein in IP experiments. IP Sample:1000 ug 293T whole cell lysate/extract. A. 20 ug 293T whole cell lysate/extract. B. Control with 2.5 ug of preimmune rabbit IgG. C. Immunoprecipitation of LDB1 protein by 2.5 ug of LDB1 antibody . 12% SDS-PAGE. The immunoprecipitated LDB1 protein was detected by LDB1antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- LDB1antibody immunoprecipitates LDB1 protein in IP experiments. IP Sample:1000 ug 293T whole cell lysate/extract. A. 20 ug 293T whole cell lysate/extract. B. Control with 2.5 ug of preimmune rabbit IgG. C. Immunoprecipitation of LDB1 protein by 2.5 ug of LDB1 antibody . 12% SDS-PAGE. The immunoprecipitated LDB1 protein was detected by LDB1antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC of paraffin-embedded U87 Xenograft using LDB1 antibody at 1:100 dilution.
