GTX48750

antibody from GeneTex

Targeting: LDB1 CLIM2, NLI

ELISA

Chromatin Immunoprecipitation

Antibody data

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Validation data

LDB1 protein structure - GTX48750 shown in red.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Western blot using GeneTex's affinity purified anti-LDB1 antibody shows detection of LDB1 protein (arrowhead) in Jurkat whole cell lysate. Approximately 30 µg of lysate was loaded prior to separation and transfer to nitrocellulose. Primary antibody was used at a 1:1,800 dilution in 5% BLOTTO in PBS reacted overnight at 4°C. The membrane was washed and reacted with a 1:20,000 dilution of DyLight800 conjugated goat anti-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). Fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Western blot using GeneTex's affinity purified anti-LDB1 antibody shows detection of LDB1 protein (arrowhead) in Jurkat whole cell lysate. Approximately 30 ?g of lysate was loaded prior to separation and transfer to nitrocellulose. Primary antibody was used at a 1:1,800 dilution in 5% BLOTTO in PBS reacted overnight at 4¢XC. The membrane was washed and reacted with a 1:20,000 dilution of DyLight800 conjugated goat anti-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). Fluorescence image was captured using the Odyssey? Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.