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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA5-21577 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LDB1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, Mouse brain. Predicted reactivity: Mouse (99%), Rat (99%), Xenopus laevis (97%), Pig (98%), Chicken (97%), Rhesus Monkey (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.0 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Transcription factor Isl1 is dispensable for the development of the mouse prosensory region.
He D, Guo R, Zheng D, Xu M, Li P, Guo L, Gan L
Cytotechnology 2020 Jun;72(3):407-414
Cytotechnology 2020 Jun;72(3):407-414
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of LDB1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: LDB1 Polyclonal Antibody (Product # PA5-21577) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- LDB1antibody immunoprecipitates LDB1 protein in IP experiments. IP Sample: 1,000 µg 293T whole cell lysate/extract. A. 20 µg 293T whole cell lysate/extract. B. Control with 2.5 µg of preimmune rabbit IgG. C. Immunoprecipitation of LDB1 protein by 2.5 µg of LDB1 antibody (Product # PA5-21577). 12% SDS-PAGE. The immunoprecipitated LDB1 protein was detected by LDB1antibody (Product # PA5-21577) diluted at 1:1,000.
