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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-21577 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LDB1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, Mouse brain.
- Concentration
- 1.0 mg/mL
Submitted references Transcription factor Isl1 is dispensable for the development of the mouse prosensory region.
He D, Guo R, Zheng D, Xu M, Li P, Guo L, Gan L
Cytotechnology 2020 Jun;72(3):407-414
Cytotechnology 2020 Jun;72(3):407-414
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LDB1 using A) 30 µg 293T whole cell extract (B) 30 µg A431 whole cell extract (C) 30 µg HeLa whole cell extract and D) 30 µg HepG2 whole cell extract. Samples were loaded onto a 10% SDS-PAGE gel and probed with a LDB1 polyclonal antibody (Product # PA5-21577) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of LDB1 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a LDB1 Polyclonal Antibody (Product # PA5-21577) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using LDB1 Polyclonal Antibody (Product # PA5-21577). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 10% SDS PAGE. LDB1 Polyclonal Antibody (Product # PA5-21577) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using LDB1 Polyclonal Antibody (Product # PA5-21577). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDB1 Polyclonal Antibody (Product # PA5-21577) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of LDB1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: LDB1 Polyclonal Antibody (Product # PA5-21577) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded U87 Xenograft, using LDB1 (Product # PA5-21577) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- LDB1 Polyclonal Antibody detects LDB1 protein at nucleus on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. LDB1 Polyclonal Antibody (Product # PA5-21577) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- LDB1 Polyclonal Antibody detects LDB1 protein at nucleus on rat middle brain by immunohistochemical analysis. Sample: Paraffin-embedded rat middle brain. LDB1 Polyclonal Antibody (Product # PA5-21577) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- LDB1antibody immunoprecipitates LDB1 protein in IP experiments. IP Sample: 1,000 µg 293T whole cell lysate/extract. A. 20 µg 293T whole cell lysate/extract. B. Control with 2.5 µg of preimmune rabbit IgG. C. Immunoprecipitation of LDB1 protein by 2.5 µg of LDB1 antibody (Product # PA5-21577). 12% SDS-PAGE. The immunoprecipitated LDB1 protein was detected by LDB1antibody (Product # PA5-21577) diluted at 1:1,000.
