Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-54416 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMAP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: ASGMVAPMAM PAGYMGGMQA SMMGVPNGMM TTQQAGYMAG MAAMPQTVYG VQPAQQLQWN LTQMTQQMAG MNFYGANGMM NYGQSMSGGN GQAANQTLSP QMWK Highest antigen sequence identity to the following orthologs: Mouse - 96%, Rat - 94%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SMAP2 in mouse cell line NIH-3T3 and rat cell line NBT-II using a SMAP2 Polyclonal Antibody (Product # PA5-54416).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SMAP2 in human cell lines PC-3 and A-549 using a SMAP2 Polyclonal Antibody (Product # PA5-54416). Corresponding SMAP2 RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of SMAP2 in human cell line A-431 shows positivity in cytoplasm & nucleus but excluded from the nucleoli. Samples were probed using a SMAP2 Polyclonal Antibody (Product # PA5-54416).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SMAP2 in human lymph node shows strong positivity in cells outside the germinal center. Samples were probed using a SMAP2 Polyclonal Antibody (Product # PA5-54416).