MA3-076

antibody from Invitrogen Antibodies

Targeting: TAF5 TAF2D, TAFII100

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MA3-076

Provider

Invitrogen Antibodies

Product name

TAF5 Monoclonal Antibody (1TA-1C2)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

MA3-076 detects TATA-Binding Protein (TBP) associated factor 5 from human samples.

Antibody clone number

1TA-1C2

Concentration

Conc. Not Determined

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TAF5 was performed by loading 20 µg of the indicated nuclear lysates and 5 µL of PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-Glycine polyacrylamide gel (Product # WT4202BX10). Proteins were transferred to a nitrocellulose membrane using the G2 Blotter (Product # 62288), and blocked with 5% Milk in TBST for 1 hour at room temperature. TAF5 was detected at 100 kDa using a TAF5 mouse monoclonal antibody (Product # MA3-076) at a dilution of 1:500 in blocking buffer overnight at 4°C on a rocking platform, followed by a Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177) at a dilution of 1:1000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34078).