MA3-076

antibody from Invitrogen Antibodies

Targeting: TAF5 TAF2D, TAFII100

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MA3-076

Provider

Invitrogen Antibodies

Product name

TAF5 Monoclonal Antibody (1TA-1C2)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

MA3-076 detects TATA-Binding Protein (TBP) associated factor 5 from human samples. MA3-076 has been successfully used in Western blot application. The MA3-076 immunogen is a synthetic peptide coupled to ovalalbumin, aa 743 to aa 764.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

1TA-1C2

Vial size

50 µL

Concentration

Conc. Not Determined

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TAF5 was performed by loading 20 µg of the indicated nuclear lysates and 5 µL of PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-Glycine polyacrylamide gel (Product # WT4202BX10). Proteins were transferred to a nitrocellulose membrane using the G2 Blotter (Product # 62288), and blocked with 5% Milk in TBST for 1 hour at room temperature. TAF5 was detected at 100 kDa using a TAF5 mouse monoclonal antibody (Product # MA3-076) at a dilution of 1:500 in blocking buffer overnight at 4°C on a rocking platform, followed by a Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177) at a dilution of 1:1000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34078).