A303-685A

antibody from Invitrogen Antibodies

Targeting: TAF5 TAF2D, TAFII100

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A303-685A

Provider

Invitrogen Antibodies

Product name

TAF5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human TAF5 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-TAF5 antibody A303-685A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. TAF5 was also immunoprecipitated by rabbit anti-TAF5 antibodies A303-686A and A303-687A, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human TAF5 by immunohistochemistry. Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- TAF5 (Cat. No. A303-685A Lot1, left image, A303-686A Lot1, middle image and Cat. No. A303-687A Lot1, right image) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB. Counterstain: Hematoxylin (blue).