NBP2-20132

antibody from Novus Biologicals

Targeting: RBP1 CRABP-I, CRBP, CRBP1, CRBPI, RBPC

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-20132

Provider

Novus Biologicals

Product name

Rabbit Polyclonal Retinol Binding Protein RBP Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

Chrysin Ameliorates Malfunction of Retinoid Visual Cycle through Blocking Activation of AGE-RAGE-ER Stress in Glucose-Stimulated Retinal Pigment Epithelial Cells and Diabetic Eyes.

Kang MK, Lee EJ, Kim YH, Kim DY, Oh H, Kim SI, Kang YH
Nutrients 2018 Aug 8;10(8)

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Retinol Binding Protein RBP Antibody [NBP2-20132] - CRBP I antibody detects RBP1 protein by Western blot analysis. A. 30 ug NIH-3T3 whole cell lysate/extractB. 30 ug C2C12 whole cell lysate/extract12 % SDS-PAGECRBP I antibody dilution: 1:5000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Retinol Binding Protein RBP Antibody [NBP2-20132] - Sample (30 ug of whole cell lysate) A: H1299 15% SDS PAGE gel, diluted at 1:5000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Retinol Binding Protein RBP Antibody [NBP2-20132] - Elevation of retinal tissue induction of visual cycle enzymes stimulated by retinoic acid 6 (STRA6, C) in chrysin-treated mice. The db/db mice were orally supplemented with 10 mg/kg of chrysin daily for 10 weeks. The db/m mice were introduced as control animals. Mouse retinal tissue extracts were subject to Western blot analysis with a primary antibody against each target protein of LRAT, RDH5, cellular retinol binding protein (CRBP), CRALBP, IRBP, or STRA6. beta-actin protein was used as an internal control. Bar graphs (mean +- SEM, n = nine independent experiments) in the bottom or right panels represent densitometric results of upper blot bands. * Values in bar graphs indicate a significant difference at p

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Retinol Binding Protein RBP Antibody [NBP2-20132] - Human colon, at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min