PA5-28713

antibody from Invitrogen Antibodies

Targeting: RBP1 CRABP-I, CRBP, CRBP1, CRBPI, RBPC

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28713

Provider

Invitrogen Antibodies

Product name

RBP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: H1299, NIH-3T3, C2C12.

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CRBP I using 30 µg of H1299 lysate. Samples were loaded onto a 15% SDS-PAGE gel and probed with a CRBP I polyclonal antibody (Product # PA5-28713) at a dilution of 1:5000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using RBP1 Polyclonal Antibody (Product # PA5-28713). Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with RBP1 Polyclonal Antibody (Product # PA5-28713) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CRBP I antibody detects RBP1 protein by western blot analysis. A. 30 µg NIH-3T3 whole cell lysate/extract. B. 30 µg C2C12 whole cell lysate/extract.12% SDS-PAGE. CRBP I antibody RBP1 Polyclonal Antibody (Product # PA5-28713) dilution: 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of CRBP I in methanol-fixed H1299 cells using a CRBP I polyclonal antibody (Product # PA5-28713) at a 1:500 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RBP1 Polyclonal Antibody detects RBP1 protein at cytoplasm by immunofluorescent analysis. Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RBP1 stained by RBP1 Polyclonal Antibody (Product # PA5-28713) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10 µm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RBP1 Polyclonal Antibody detects RBP1 protein at cytoplasm by immunofluorescent analysis. Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RBP1 stained by RBP1 Polyclonal Antibody (Product # PA5-28713) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human colon, using CRBP I (Product # PA5-28713) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.