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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
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- Product number
- PA5-28713 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RBP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: H1299, NIH-3T3, C2C12. Predicted reactivity: Mouse (100%), Rat (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CRBP I using 30 µg of H1299 lysate. Samples were loaded onto a 15% SDS-PAGE gel and probed with a CRBP I polyclonal antibody (Product # PA5-28713) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RBP1 Polyclonal Antibody (Product # PA5-28713). Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with RBP1 Polyclonal Antibody (Product # PA5-28713) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CRBP I antibody detects RBP1 protein by western blot analysis. A. 30 µg NIH-3T3 whole cell lysate/extract. B. 30 µg C2C12 whole cell lysate/extract.12% SDS-PAGE. CRBP I antibody RBP1 Polyclonal Antibody (Product # PA5-28713) dilution: 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CRBP I in methanol-fixed H1299 cells using a CRBP I polyclonal antibody (Product # PA5-28713) at a 1:500 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RBP1 Polyclonal Antibody detects RBP1 protein at cytoplasm by immunofluorescent analysis. Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RBP1 stained by RBP1 Polyclonal Antibody (Product # PA5-28713) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10 µm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RBP1 Polyclonal Antibody detects RBP1 protein at cytoplasm by immunofluorescent analysis. Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RBP1 stained by RBP1 Polyclonal Antibody (Product # PA5-28713) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon, using CRBP I (Product # PA5-28713) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
