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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [1]
- Flow cytometry [1]
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- Product number
- NB100-1494 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1494, RRID:AB_2238376
- Product name
- Goat Polyclonal Retinol Binding Protein RBP Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Cellular retinol-binding protein I, a regulator of breast epithelial retinoic acid receptor activity, cell differentiation, and tumorigenicity.
Farias EF, Ong DE, Ghyselinck NB, Nakajo S, Kuppumbatti YS, Mira y Lopez R
Journal of the National Cancer Institute 2005 Jan 5;97(1):21-9
Journal of the National Cancer Institute 2005 Jan 5;97(1):21-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Retinol Binding Protein RBP Antibody [NB100-1494] - Staining of NIH3T3 embryonic mouse cell lysate with antibody at 2 ug/mL (A) and U251 cell lysate with antibody at 0.5 ug/mL (B). 35 ug protein each in RIPA buffer.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Retinol Binding Protein RBP Antibody [NB100-1494] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
