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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- TA302129 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Antibody against MYST2 (Center)
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Antibody against MYST2 (Center)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- KAT7
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 0.25 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HBO1/MYST2 Antibody (Center) (Cat. #TA302129) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the HBO1/MYST2 antibody detected the HBO1/MYST2 protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of Hela cell stained with HBO1/MYST2 Antibody (Center)(Cat#TA302129). Hela cells were incubated with HBO1/MYST2 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . HBO1/MYST2 immunoreactivity is localized to nucleus significantly.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human testis tissue reacted with HBO1/MYST2 antibody (Center) (Cat.#TA302129), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- IHC
