sc-9447

antibody from Santa Cruz Biotechnology

Targeting: MTA2 MTA1-L1, MTA1L1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-9447

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-9447, RRID:AB_2146965

Product name

Anti-MTA2

Antibody type

Polyclonal (Antigen purified)

Antigen

Synthetic peptide

Reactivity

Human

Host

Goat

References

Submitted references

Sumoylation regulates interaction of FOG1 with C-terminal-binding protein (CTBP).

Snow JW, Kim J, Currie CR, Xu J, Orkin SH
The Journal of biological chemistry 2010 Sep 3;285(36):28064-75

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NuRD mediates activating and repressive functions of GATA-1 and FOG-1 during blood development.

Miccio A, Wang Y, Hong W, Gregory GD, Wang H, Yu X, Choi JK, Shelat S, Tong W, Poncz M, Blobel GA
The EMBO journal 2010 Jan 20;29(2):442-56

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Chromatin architecture and transcription factor binding regulate expression of erythrocyte membrane protein genes.

Steiner LA, Maksimova Y, Schulz V, Wong C, Raha D, Mahajan MC, Weissman SM, Gallagher PG
Molecular and cellular biology 2009 Oct;29(20):5399-412

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Translational isoforms of FOG1 regulate GATA1-interacting complexes.

Snow JW, Orkin SH
The Journal of biological chemistry 2009 Oct 23;284(43):29310-9

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Nanog and Oct4 associate with unique transcriptional repression complexes in embryonic stem cells

Jiancong Liang, Ma Wan, Yi Zhang, Peili Gu, Huawei Xin, Sung Yun Jung, Jun Qin, Jiemin Wong, Austin J. Cooney, Dan Liu, Zhou Songyang
Nature Cell Biology 2008 May;10(6):731-739

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Active gene repression by the Egr2.NAB complex during peripheral nerve myelination.

Mager GM, Ward RM, Srinivasan R, Jang SW, Wrabetz L, Svaren J
The Journal of biological chemistry 2008 Jun 27;283(26):18187-97

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The zinc finger and C-terminal domains of MTA proteins are required for FOG-2-mediated transcriptional repression via the NuRD complex.

Roche AE, Bassett BJ, Samant SA, Hong W, Blobel GA, Svensson EC
Journal of molecular and cellular cardiology 2008 Feb;44(2):352-60

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MBD2 is a critical component of a methyl cytosine-binding protein complex isolated from primary erythroid cells.

Kransdorf EP, Wang SZ, Zhu SZ, Langston TB, Rupon JW, Ginder GD
Blood 2006 Oct 15;108(8):2836-45

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FOG-1 recruits the NuRD repressor complex to mediate transcriptional repression by GATA-1.

Hong W, Nakazawa M, Chen YY, Kori R, Vakoc CR, Rakowski C, Blobel GA
The EMBO journal 2005 Jul 6;24(13):2367-78

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

No bands detected.

Primary Ab dilution

1:500

Secondary Ab dilution

1:2000

Lane 1

Marker [kDa]: 220, 112, 84, 47, 32, 26, 16.8

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 75

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human small intestine shows strong nuclear positivity in glandular cells.

Validation comment

Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.